1x minimum essential medium mem vitamin solution (Thermo Fisher)

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Thermo Fisher 1x minimum essential medium mem vitamin solution
1x Minimum Essential Medium Mem Vitamin Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+minimum+essential+medium+mem+vitamin+solution/LB+Medium/pm39955738-61-92-99
Average 96 stars, based on 1 article reviews

1x minimum essential medium mem vitamin solution - by Bioz Stars, 2026-09

96/100 stars

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Cell Culture:

Article Title: Argonaute‐2 Promotes miR‐18a Entry in Human Brain Endothelial Cells
Article Snippet: .. BEC were cultured in RPMI 1640 growth media (Mediatech Inc) supplemented with 100 ng/mL EC growth supplement (Millipore), 10 mmol/L N-2-hy-droxyethylpiperazine-N-2-ethanesulfonic acid (HEPES) (Invitrogen), 24 mmol/ L sodium pyruvate (Invitrogen), 300 U heparin (SigmaAldrich), 1x minimum essential medium (MEM) vitamin solution (Invitrogen), 1x MEM nonessential amino acids (Mediatech Inc), 1% penicillin/streptomycin (Invitrogen) and 10% fetal calf serum (FCS) (Omega Scientific Inc) in a 37°C incubator with 5% CO2. .. After reaching 80% confluency, cells were labeled with CD31/platelet-EC adhesion molecule-1labeled magnetic Dyna beads (Invitrogen) and purified by Mini MACS magnetic cell sorting (Miltenyl Biotec, GmbH).

Clinical Proteomics:

Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.
Article Snippet: Sertoli cells were expanded on tissue culture-treated plastic in Sertoli Cell Medium (Sciencell, 4521). .. SSCs were expanded on a sulfated dextran-4-armed polyethylene glycol (starPEG) hydrogel matrix functionalized with vitronectin and fibronectin type II domain (FN2) peptide motifs (denovoMATRIX, GmbH) in Human Plasma-Like Medium (HPLM), 15% Cell Therapy Systems (CTS) KnockOut Serum Replacement (SR) XenoFree supplement (Gibco, 12 618 012), 1 μM vitamin C (Millipore Sigma, A4403), 1 μM vitamin E (Millipore Sigma, T1157), 10 μg mL−1 biotin (Millipore Sigma, B4639), 60 ng mL−1 progesterone (Millipore Sigma, P8783), 60 μM putrescine (Millipore Sigma, P5780), 30 μg mL−1 pyruvate (Millipore Sigma, S8636), 30 ng mL−1 β-estradiol (Sigma, E2758), 1X Minimum Essential Medium (MEM) Vitamin Solution (Gibco, 11 120 052), 1X GlutaMAX Supplement (Gibco, 35 050 061), 1 μL mL−1 DL-lactate (Millipore Sigma, L4263), 50 μM β-mercaptoethanol (Gibco, 31350- 010), 15 ng mL−1 Glial Cell-Derived Neurotrophic Factor (GDNF, Peprotech, AF-450-010), 10 ng mL−1 heat stable FGF2, and either 10 ng mL−1 EGF or 100 nM Akt pathway inhibitor MK2206 Adv. ..

Knock-Out:

Modification:

Article Title: TRPM8 contributes to sex dimorphism by promoting recovery of normal sensitivity in a mouse model of chronic migraine
Article Snippet: .. Cells were kept in a hormone-free culture medium consisting of Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12) without Phenol Red (11039021, Thermo Fisher Scientific), 1X Minimum Essential Medium (MEM) vitamin solution (11120052, Thermo Fisher Scientific), 1% penicillin/streptomycin (15140-022, Thermo Fisher Scientific) and home-made μg/ml N 2 -containing insulin (I2643, Merck), 0.1 mM putrescine hydrochloride (P7505, Merck), 3 nM sodium selenite (S5261 Merck), 100 μg/ml transferrin (T2872, Molecular Probes, Eugene, USA), 25 ng/ml NGF (G5141, Promega, Madison, USA) and 25 ng/ml hGDNF (450-10, Peprotech, London, UK). .. Non-ratiometric calcium imaging experiments were conducted with the fluorescent indicator fluo4-AM (F14201, Thermo Fisher Scientific).

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